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Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
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Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

DOI:
10.3791/50717-v

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16:41 min

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November 05, 2013

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,
1Department of Biochemistry,University of Geneva

Chapters

  • 00:05Title
  • 02:42OxyBurst Green (OBG)-Coating of Beads
  • 05:43Visualization and Qualitative Measurement of ROS Production in Phagosomes
  • 08:48Quantitative and Medium-throughput Measurement of Intracellular Superoxide Production
  • 10:30Quantitative and Medium-throughput Measurement of Extracellular H2O2 Production
  • 12:04Results: OxyBurst Green (OBG), Dihydroethidium (DHE) and Amplex UltraRed (AUR) Assays can Detect and Measure ROS in Dictyostelium
  • 15:54Conclusion

Summary

Automatic Translation

Automatic Translation

We adapted a set of protocols for the measurement of reactive oxygen species (ROS) that can be applied in various amoeba and mammalian cellular models for qualitative and quantitative studies.

Tags

Keywords: Reactive Oxygen SpeciesROSOxyBurst GreenDihydroethidiumDHEAmplex UltraRedAURDictyosteliumPhagocytesLipopolysaccharideLPSSuperoxideHydrogen PeroxideFluorescence MicroscopyQuantitation

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