Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Shuai Nie; Elena Benito-Pe&#241;a; Huaibin Zhang; Yue Wu; David R. Walt

doi:10.3791/50726

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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

11,616 Views

•

08:50 min

•

October 10, 2013

DOI:

10.3791/50726-v

DOI:

10.3791/50726-v

•

08:50 min

October 10, 2013

•

1 Views

Shuai Nie¹, Elena Benito-Peña^1,2, Huaibin Zhang¹, Yue Wu³, David R. Walt¹

¹Department of Chemistry,Tufts University, ²Department of Analytical Chemistry,Complutense University (Spain), ³Department of Electrical and Computer Engineering,Tufts University

Summary

We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.