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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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JoVE Journal Chemistry
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

DOI:
10.3791/50726-v

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08:50 min

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October 10, 2013

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, , , ,
1Department of Chemistry,Tufts University, 2Department of Analytical Chemistry,Complutense University (Spain), 3Department of Electrical and Computer Engineering,Tufts University

Chapters

  • 00:05Title
  • 01:20Microspheres Encoding
  • 02:42Preparation of Protein-capture Microspheres
  • 03:48Fiber-optic Microarray Assembly
  • 05:50Saliva Sample Analysis Using Microspheres Microarray
  • 07:10Results: Human Salivary Protein Analysis
  • 08:22Conclusion

Summary

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Automatic Translation

We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.

Tags

Keywords: MultiplexedFluorescentMicroarraySalivary ProteinAnalysisPolymer MicrospheresFiber-optic BundlesImmunoassaySuspension ArrayEncodingFluorescence MicroscopySandwich AssayBiotinylated Detection AntibodiesStreptavidinR-phycoerythrin

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