Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

The overall goal of this procedure is to analyze multiple proteins in human saliva samples using microsphere based fluorescent arrays. First, encode the microspheres with different amounts of two fluorescent dyes. Couple the capture antibodies for different proteins onto the encoded microspheres.

Then assemble the multiplexed protein microarray on the fiber optic bundle using the sandwiched immunoassays on the assembled microarrays assay for multiple proteins in the test saliva samples. Ultimately, analysis by fluorescence microscopy can measure the signal responses of different proteins in the saliva samples. The main advantage of this technique over existing methods like Eliza's is that it enables the simultaneous analysis of multiple proteins in complex biofluids.