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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Immunology and Infection
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JoVE Journal Immunology and Infection
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

DOI:
10.3791/50730-v

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08:31 min

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October 15, 2013

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, , ,
1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children,University of British Columbia

Chapters

  • 00:05Title
  • 01:08Protein Extraction
  • 02:41First-dimensional Isoelectric Focusing
  • 04:06Second-dimension SDS Polyacrylamide Gel Electrophoresis and Sliver Staining
  • 07:14Representative Protein Extraction Results
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.

Tags

Protein ExtractionBurkholderia Species2-D Gel ElectrophoresisProteomicsIsoelectric FocusingSDS-PAGESilver Staining

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