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JoVE Journal
Immunology and Infection

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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
 

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Article DOI: 10.3791/50730
October 15th, 2013

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Summary October 15th, 2013

Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.

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