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December 09, 2013
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Primary disassociated embryonic hippocampal neuronal cultures are useful for investigating the signaling mechanisms involved in neuron death. Sexing the embryos before the isolation and dissociation of the hippocampus allows the preparation of separate male and female cultures, which enables the researcher to identify and investigate sex-specific cell signaling.
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Cite this Article
Fairbanks, S. L., Vest, R., Verma, S., Traystman, R. J., Herson, P. S. Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways. J. Vis. Exp. (82), e50758, doi:10.3791/50758 (2013).
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