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December 09, 2013
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This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.
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Cite this Article
Mariol, M., Walter, L., Bellemin, S., Gieseler, K. A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome. J. Vis. Exp. (82), e50773, doi:10.3791/50773 (2013).
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