Uv Irradiation
Uv Irradiation - Related Videos
03:40
UV Irradiation: A Method to Integrate Extrachromosomal Arrays
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2023
Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene. UV Irradiation can induce chromosomal integration of the array and more stable transgene transmission.
03:54
An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells
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2025
This video demonstrates a method to induce apoptosis in the Jurkat cells — a human T lymphocyte leukemia cell line. The cells are exposed to ultraviolet C (UVC) radiation, which causes extensive DNA damage and leads to apoptotic cell death.
08:48
Selective Area Modification of Silicon Surface Wettability by Pulsed UV Laser Irradiation in Liquid Environment
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Cited by 1 •
2015
We report on a process of in situ alteration of HF treated Si (001) surface into a hydrophilic or hydrophobic state by irradiating samples in microfluidic chambers filled with H2O2/H2O solution (0.01%-0.5%) or methanol solutions using pulsed UV laser of a relative low pulse fluence.
04:15
A Mouse Model of Fatigue Induced by Peripheral Irradiation
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Cited by 18 •
2017
We describe a method using targeted peripheral irradiation to induce fatigue-like behavior in mice. The selected non-lethal irradiation dose leads to a week-long reduction in voluntary wheel-running activity.
10:09
Planarian Immobilization, Partial Irradiation, and Tissue Transplantation
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Cited by 20 •
2012
An effective method for grafting tissue of defined and consistent size between planaria is described. Also included is a description of how the immobilization technique used for transplantation can be adapted, in conjunction with lead shields, for the partial irradiation of live animals.
FAQs
These three variables determine the delivered UV dose and therefore the extent of biological or material change. Wavelength affects which processes are initiated, while intensity and exposure time control how much energy reaches the target. UV-C is particularly important for nucleic-acid damage, but changing any of these parameters can alter whether the outcome is inactivation, crosslinking, or polymer modification.
UV-C can produce thymine dimers within nucleic acids, creating lesions that interfere with replication and transcription. When these essential processes are disrupted, microorganisms may lose the ability to reproduce or remain biologically functional. This mechanism explains why UV-C is useful for sterilization-related work, while also emphasizing that exposure can damage any nucleic-acid-containing biological material in the treatment area.
The same photochemical effects that support microbial inactivation or biomaterial processing can harm living cells and tissues. Excessive exposure may produce unwanted biological damage, whereas insufficient exposure may fail to achieve the intended modification. Bioengineering studies therefore select wavelength, intensity, and exposure time according to the target outcome, especially when designing cell-compatible materials or tissue-engineering systems.
A procedure should begin by identifying the target material or organism and the intended outcome, such as sterilization, crosslinking, or polymer modification. The operator then selects an appropriate wavelength, intensity, and exposure time, with particular attention to dose when living cells or tissues are involved. These choices determine whether the treatment produces useful modification or unwanted damage.
In biomaterials research, controlled exposure can drive photochemical crosslinking, a process that links material components to alter the structure of a polymer or hydrogel. The resulting modification supports investigation of material systems used in tissue engineering and related bioengineering work. Because exposure conditions affect the extent of change, dose selection remains important for obtaining a controlled material outcome.
Beyond surface and equipment sterilization, UV irradiation supports controlled modification of polymers and hydrogels, photochemical crosslinking of biomaterials, and research connected with tissue engineering. It also contributes to biomedical device development by enabling deliberate changes in material behavior or surface-related properties. These applications rely on controlling exposure so that the desired material change is achieved without unnecessary biological damage.