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Udarbejdelse af Primary neuroner til visualisering neurites i frossen hydreret tilstand vha. Cryo-Electron Tomography
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1Department of Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Neuroscience,University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Chapters
- 00:05Title
- 01:19Preparing Dishes with EM Grids
- 03:43Preparing and Plating Primary Neurons on EM Grids
- 04:45Vitrifying Neurons on EM Grids
- 08:05Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
- 09:32Conclusion
For at bevare neuronale processer til ultrastrukturelle analyse beskriver vi en protokol til galvanisering af primære neuroner på elektronmikroskopi gitre efterfulgt af flash frysning, hvilket giver prøver suspenderet i et lag af glasagtig is. Disse prøver kan undersøges med en cryo-elektron mikroskop til at visualisere strukturer på nanometer skala.
