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Immunology and Infection

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Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
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Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Article DOI: 10.3791/50788 • 10:40 min • July 17th, 2013
July 17th, 2013 •
1, 1, 1
1Medical Microbiology, University Medical Center Utrecht

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Summary
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We present methods to study the effect of PSMs and other toxins secreted by Staphylococcus aureus on neutrophils using flow cytometry and fluorescence microscopy.

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Staphylococcus Aureus Neutrophils Flow Cytometry Time-lapse Microscopy Phenol Soluble Modulins (PSMs) Toxins Density Gradient Centrifugation Calcium Mobilization Intracellular Calcium Concentration Flow Cytometry Experiment Fluorescence Dye Neutrophil Activation PSM Concentration Inhibitors Reporter Fusions GFP Promoter Of The PSMα Operon Phagocytosis Expression Induction
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