Journal
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Immunology and Infection
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Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

DOI:
10.3791/50788-v

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10:40 min

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July 17, 2013

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, ,
1Medical Microbiology,University Medical Center Utrecht

Chapters

  • 00:05Title
  • 01:32Density Gradient Separation of Polymorphonuclear Neutrophils (PMNs) from Human Blood
  • 04:31Flow Cytometric for Assessment of Calcium Mobilization in Human PMNs
  • 06:40Fluorescence Microscopy Analysis of Bacteria GFP Expression after Phagocytosis by PMNs
  • 08:51Results: Representative Analyses of PSMα Activation in Human PMNs
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

We present methods to study the effect of PSMs and other toxins secreted by Staphylococcus aureus on neutrophils using flow cytometry and fluorescence microscopy.

Tags

Keywords: Staphylococcus AureusNeutrophilsFlow CytometryTime-lapse MicroscopyPhenol-soluble Modulins (PSMs)Calcium MobilizationReporter FusionsGFP

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