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In vivo 19F MRI for Cell Tracking
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JoVE Journal Medicine
In vivo 19F MRI for Cell Tracking

In vivo 19F MRI for Cell Tracking

DOI:
10.3791/50802-v

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10:05 min

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November 25, 2013

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, , , , , ,
1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences,Radboud University Medical Center, 2In-Vivo-NMR Laboratory,Max Planck Institute for Neurological Research, 3German Center for Neurodegenerative Diseases (DZNE)

Chapters

  • 00:05Title
  • 02:00Cell Labeling (Standard Protocol with Coincubation)
  • 02:57Determination of 19F/cell using 19F NMR
  • 04:17Imaging and Mouse Preparation
  • 05:29B1 Correction
  • 07:44Image Processing
  • 08:45Results: 19F-labeled Cells Homing to a Draining Lymph Node
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.

Tags

Keywords: 19F MRICell TrackingNoninvasiveQuantitativeMurine Immune CellsCell LabelingImaging ParametersImage Processing

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