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Maus embryonalen Entwicklung in einem Serum-freien Whole Embryo Culture-System
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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Maus embryonalen Entwicklung in einem Serum-freien Whole Embryo Culture-System

Article DOI: 10.3791/50803 • 08:55 min
March 1st, 2014 •
1, 1,2
1Neuroscience Division of the Biomedical & Health Sciences Institute, University of Georgia, 2Department of Cellular Biology, University of Georgia

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March 1st, 2014

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Serum in Embryokulturen genutzt enthält unbekannte Komponenten, die das Ergebnis von Experimenten, vor allem in Studien mit Signal Wechselwirkungen beeinflussen können. Hier verwendeten wir eine Serum-freien Kultursystem mit Sauerstoff angereichert und zeigen, dass Mitte der Schwangerschaft Maus-Embryonen für 16-40 h kultiviert morphologische Entwicklung vergleichbar zu entwickelnden Embryonen in utero.

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