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总钙在神经元的电子探针X射线显微分析测量
JoVE Journal
Neuroscience
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Neuroscience
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
Please note that all translations are automatically generated.
Click here for the English version.
总钙在神经元的电子探针X射线显微分析测量
DOI:
10.3791/50807-v
•
11:42 min
•
November 20, 2013
•
Natalia B. Pivovarova
,
S. Brian Andrews
1
Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke
,
National Institutes of Health
Chapters
00:05
Title
01:29
Rapid Freezing and Cryosectioning of Cells Cultured on Coverslips
03:33
Cryotransfer of Specimens to the Electron Microscope
05:00
Freeze Drying and Imaging of Cells and Organelles
06:35
Acquisition of Mitochondrial X-ray Spectra
07:44
Qualitative and Quantitative Analysis of X-ray Spectra
09:33
Results: Mitochondria Sequester Calcium in Response to Glutamate Receptor Activation
11:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍cryoanalytical电子显微镜对总钙含量和分布在生理上定义的生物标本亚细胞分辨率的定量测量中的应用。
Tags
Electron Probe X-ray Microanalysis
EPMA
Intracellular Elemental Content
Intramitochondrial Calcium
Cryofixation
Cryosections
Energy-dispersive X-ray Spectrometry
EDX
Neurodegenerative Diseases
Excitotoxic Injury
Ischemia Resistance
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