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总钙在神经元的电子探针X射线显微分析测量
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
DOI:

11:42 min

November 20, 2013

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Chapters

  • 00:05Title
  • 01:29Rapid Freezing and Cryosectioning of Cells Cultured on Coverslips
  • 03:33Cryotransfer of Specimens to the Electron Microscope
  • 05:00Freeze Drying and Imaging of Cells and Organelles
  • 06:35Acquisition of Mitochondrial X-ray Spectra
  • 07:44Qualitative and Quantitative Analysis of X-ray Spectra
  • 09:33Results: Mitochondria Sequester Calcium in Response to Glutamate Receptor Activation
  • 11:17Conclusion

Summary

Automatic Translation

本文介绍cryoanalytical电子显微镜对总钙含量和分布在生理上定义的生物标本亚细胞分辨率的定量测量中的应用。

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