Fluorescent Azide
Fluorescent Azide - Related Videos
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
0 Views •
Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
01:22
Preparation of 1° Amines: Azide Synthesis
0 Views •
2023
Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used. Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
09:35
Preparation of a Corannulene-functionalized Hexahelicene by Copper(I)-catalyzed Alkyne-azide Cycloaddition of Nonplanar Polyaromatic Units
0 Views •
Cited by 1 •
2016
Here, we present a protocol to synthesize a complex organic compound comprised of three nonplanar polyaromatic units, assembled easily with reasonable yields.
09:06
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
0 Views •
Cited by 3 •
2016
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
03:03
Fluorescent Reporter-Based Paralysis Assay: A Technique to Assess Age-Associated Progressive Formation of Polyglutamine Fluorescent Reporter and Associated Paralysis in Caenorhabditis elegans
0 Views •
2025
This video demonstrates an in vivo imaging-based assay to determine proteostasis decline due to aging. The assay uses Caenorhabditis elegans expressing polyglutamine YFP-fusion protein in body wall muscles to measure the age-associated aggregation of polyglutamine and induction of proteotoxicity, leading to paralysis.
FAQs
Both reaction formats connect the azide probe to an alkyne-labeled target through bioorthogonal azide-alkyne cycloaddition. Copper-catalyzed click chemistry uses copper to promote formation of the linkage, whereas strain-promoted reactions use an activated, strained alkyne instead. This distinction gives researchers alternative ways to create the same stable triazole connection while limiting interference with cellular processes.
The triazole linkage provides a stable connection between the fluorescent azide and the alkyne-labeled biomolecule. Because the attachment is formed through a bioorthogonal reaction, the probe can mark the selected target while minimizing disruption to cellular processes. Stability helps preserve the fluorescent label during subsequent visualization and signal measurement, supporting analysis of molecular localization.
Fluorescence makes the tagged target detectable through emitted light, allowing researchers to visualize where it occurs and compare signal across cells or tissues. Microscopy can reveal localization, while signal measurement provides a way to assess fluorescence intensity. Together, these readouts support spatial analysis of molecular events rather than indicating only that a target was chemically labeled.
A typical workflow begins with an alkyne-labeled biomolecule or biological target, followed by introduction of the fluorescent azide. Bioorthogonal azide-alkyne cycloaddition then forms the triazole linkage between the probe and target. The resulting fluorescent signal can be examined by microscopy or measured to determine target localization and distribution in cells or tissues.
In immunology and infection research, these probes help localize glycans, proteins, and other molecules associated with immune-cell function, pathogen attachment, and host-pathogen interactions. Their labeling capability connects molecular identity with position in a biological sample. Researchers can therefore examine where relevant targets occur and assess their spatial relationships during cellular or infectious processes.
Fluorescent azides can produce microscopy images, measurable fluorescence signals, and spatial information about tagged biomolecules. These outcomes help determine whether glycans, proteins, or other targets are present in particular cellular or tissue locations. In infection studies, the same readouts can support examination of pathogen attachment and host-pathogen interactions through their molecular distribution.