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Journal / Immunology and Infection / Brug Klik Chemistry at måle effekten af ​​Viral…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Brug Klik Chemistry at måle effekten af ​​Viral infektion på Host-celle RNA-syntese
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Brug Klik Chemistry at måle effekten af ​​Viral infektion på Host-celle RNA-syntese

Article DOI: 10.3791/50809-v • 09:35 min • August 9th, 2013
August 9th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch

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Denne metode beskriver brugen af ​​klik kemi til at måle ændringer i værtscelle transskription efter infektion med Rift Valley fever virus (RVFV) stamme MP-12. Resultaterne kan visualiseres kvalitativt via fluorescensmikroskopi eller opnået kvantitativt gennem flowcytometri. Denne metode kan tilpasses til brug med andre vira.

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Immunologi Virology Kemi infektionssygdomme biokemi genetik molekylærbiologi cellebiologi medicin medicoteknik arbovira, RNA Nuclear Transskription Genetic Rift Valley fever virus NSS transskription klik kemi MP-12 fluorescens mikroskopi flowcytometri virus proteiner immunfarvning assay
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