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酵母发光计量和<em>爪蟾</emTRPV4的分子Mechanosensitivity的>卵母细胞电生理检查

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Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Please note that all translations are automatically generated. Click here for the English version.

酵母发光计量和<em>爪蟾</emTRPV4的分子Mechanosensitivity的>卵母细胞电生理检查

DOI:

10.3791/50816-v

•

12:09 min

•

December 31, 2013

•

Jinfeng Teng, Stephen Loukin, Xinliang Zhou, Ching Kung²

¹Laboratory of Cell and Molecular Biology,University of Wisconsin – Madison, ²Department of Genetics,University of Wisconsin – Madison

Chapters

00:05Title
02:44Yeast Luminometry Method
04:23cRNA Injection
06:08Two-electrode Voltage-clamp
08:11Patch Clamp Examination of Direct Mechanosensitivity
09:41Results: Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
11:38Conclusion

Summary

Automatic Translation

以补充常用的方法来研究TRPV4的两个方法被描述：其mechanosensitivity可以通过在低渗挑战的转基因酵母的Ca ^{2 +-}水母发光蛋白发光法进行研究。它也可以用在TRPV4-RNA检测注入非洲爪蟾卵母细胞的全细胞双电极电压钳或膜片钳在对小区或切模式。

Tags

Keywords: TRPV4 Mechanosensitivity Yeast Luminometry Xenopus Oocyte Electrophysiology Calcium Signaling Patch Clamp Transgenic Expression Hereditary Bone/neuronal Pathologies

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