Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.
Alfaqeeh, S. A., Tucker, A. S. The Slice Culture Method for Following Development of Tooth Germs In Explant Culture. J. Vis. Exp. (81), e50824, doi:10.3791/50824 (2013).