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The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
JoVE Journal
Biology
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JoVE Journal Biology
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
DOI:

07:47 min

November 13, 2013

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Chapters

  • 00:05Title
  • 01:08Embryo Dissection
  • 02:07Embryo Slicing
  • 04:14Mandible Slice Culture and Lineage Tracing
  • 06:47Documenting Early Stages of Tooth Development
  • 07:22Conclusion

Summary

Automatic Translation

Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.

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