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JoVE Journal
Immunology and Infection

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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
 

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Article DOI: 10.3791/50833
December 3rd, 2013

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Summary December 3rd, 2013

These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.

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