Viral Amplification
Viral Amplification - Related Videos
09:44
Rapid Amplification of cDNA Ends
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2023
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research 2Department of Cell and Tissue Biology, 3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior knowledge of the sequence (Frohman et al.,...
10:12
Protein Misfolding Cyclic Amplification of Prions
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Cited by 7 •
2012
Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.
13:45
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
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Cited by 84 •
2011
The Virochip is a pan-viral microarray designed to simultaneously detect all known viruses as well as novel viruses on the basis of conserved sequence homology. Here we demonstrate how to run a Virochip assay to analyze clinical samples for the presence of both known and unknown viruses.
11:48
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
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Cited by 12 •
2019
We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.
02:41
Amplification of a Suicide Plasmid in a Bacterial Host
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2025
Source: Tomás, J. M., et al. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (2022).This video demonstrates the initiator-dependent amplification of a suicide plasmid in E. coli. The plasmid, which carries an antibiotic resistance gene, replicates only in the presence of an initiator protein encoded by the host. After electroporation and incubation in recovery medium, the mixture is plated on antibiotic-containing agar.
FAQs
Viral amplification depends on whether a host cell is susceptible, meaning it can support the viral cycle after attachment and entry. Once that condition is met, the virus redirects cellular machinery toward genome copying and viral-protein production. The resulting capacity to generate additional virions affects how efficiently infection can expand through connected or newly exposed cells.
The critical transition is the coordination of genome replication, viral-protein production, assembly, and release. Genome copies provide the genetic material for progeny, while viral proteins supply structural and functional components. If these activities do not proceed together, production of complete virions is limited. This sequence links intracellular replication with onward infection.
As viral material increases, infected tissues encounter more viral components and more infected cells, helping explain why amplification is associated with immune activation and tissue damage. In immunology research, tracking this relationship connects pathogen growth with host responses. The process therefore provides a framework for studying how changes in viral burden relate to infection-related biological effects.
A sample becomes easier to detect when amplification produces enough viral material for the detection method being used. The key implication is that detectability reflects not only the virus’s presence but also the amount of material generated in the sample. Controlled amplification can therefore improve access to a measurable signal during infection research.
In cell culture, researchers provide a controlled setting in which susceptible cells can support viral replication. They can then examine the increase in viral material and use the resulting system for detection studies or antiviral testing. This makes amplification experimentally useful because the process can be investigated under controlled research conditions rather than only during natural infection.
Beyond detection, controlled viral amplification supports antiviral testing, vaccine development, and host-pathogen interaction studies. Antiviral experiments can examine how candidate treatments relate to viral production, whereas vaccine research uses amplified viral material within development workflows. In host-pathogen studies, the process helps connect viral growth with cellular responses and infection-related changes.