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Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
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JoVE Journal Chemistry
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

DOI:
10.3791/50842-v

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10:23 min

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August 26, 2013

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, ,
1Department of Biophysical Chemistry, Institute for Physical Chemistry,University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart

Chapters

  • 00:05Title
  • 01:28Preparation of Homogeneous Gold Layers and Surface Immobilization of Bone Morphogenetic Protein 2
  • 03:51Surface Characterization
  • 04:45Analysis of Biological Activity
  • 06:25Results: Cellular Response to Surface-immobilized rhBMP-2
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.

Tags

Keywords: BMP-2Bone Morphogenetic ProteinSelf-assembled MonolayerCovalent ImmobilizationExtracellular MatrixOsteoblast DifferentiationIn Vitro Cell StudiesGrowth Factor Presentation

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