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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
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Biology
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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

DOI:
10.3791/50856-v

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12:43 min

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December 06, 2013

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, , , , ,
1Materials Science and Engineering,CSIRO

Chapters

  • 00:05Title
  • 01:38Dissociation of Human Embryonic Stem Cells (hESC) Bulk Cultures into Single Cells
  • 02:44Immunofluorescent Staining of Cell Surface Antigens TG30 and GCTM-2
  • 05:02Post-FACS Culture of TG30/GCTM-2 Cell Subfractions
  • 07:46Enrichment of Pluripotent hESC from TG30Hi-GCTM-2Hi Cells
  • 11:28Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population.

Tags

Keywords: Human Embryonic Stem CellsHESCCell Surface AntigensTG30GCTM-2FACSPluripotencyDifferentiationPurgingEnrichmentTeratomaTransplantation

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