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Biology

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Using Coculture to Detect Chemically Mediated Interspecies Interactions
 

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Article DOI: 10.3791/50863
October 31st, 2013

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Summary October 31st, 2013

Bacteria produce secreted compounds that have the potential to affect the physiology of their microbial neighbors. Here we describe a coculture screen that allows detection of such chemically mediated interspecies interactions by mixing soil microbes with fluorescent transcriptional reporter strains of Bacillus subtilis on solid media.

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