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Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans
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JoVE Journal Neuroscience
Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans

Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans

DOI:
10.3791/50864-v

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07:35 min

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February 04, 2014

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,
1Department of Psychiatry,Harvard Medical School, 2Mailman Research Center,McLean Hospital

Chapters

  • 00:05Title
  • 01:11Preparing the Drug Assay Plates
  • 02:37Assessing the Lethality and Developmental Delay
  • 04:53Assaying Pharyngeal Pumping
  • 05:55Assay Results from Clozapine Exposure
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

Approaches for testing the effects of antipsychotic drugs (APDs) in Caenorhabditis elegans are demonstrated. Assays are described for testing drug effects on development and viability and on pharyngeal pumping rate. These methods are also applicable for pharmacogenetic experiments with drug classes other than APDs.

Tags

Keywords: Caenorhabditis ElegansAntipsychotic DrugsPharmacogenetic StudiesDevelopmental AssayPharyngeal PumpingNeurotransmitter SynthesisSynaptic Structure And FunctionForward And Reverse Genetic ScreensChemical Genetic Screens

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