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JoVE Journal
Biology

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In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
 

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Article DOI: 10.3791/50894
February 17th, 2014

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Summary February 17th, 2014

We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.

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