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In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

DOI:
10.3791/50905-v

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10:51 min

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November 25, 2013

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, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Chapters

  • 00:05Title
  • 01:30Postnatal Electroporation
  • 03:36Preparation of Acute Brain Slice Cultures
  • 05:41Time-lapse Imaging of Neuroblast Migration
  • 06:57Analyzing Neuroblast Migration
  • 08:51Results: Time-lapse Imaging of Migrating Neuroblasts in Mouse Acute Brain Slices
  • 10:13Conclusion

Summary

Automatic Translation

Automatic Translation

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

Tags

Keywords: Subventricular ZoneNeurogenic NicheNeuroblast MigrationRostral Migratory StreamOlfactory BulbIn Vivo ElectroporationTime-lapse ImagingAcute Brain SlicesNeurogenesisBrain Repair

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