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Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

DOI:
10.3791/50926-v

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11:25 min

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December 26, 2013

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, , , ,
1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095,Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique,Hôpital de la Timone

Chapters

  • 00:05Title
  • 01:39Preparation of Human CD14+ Monocytes
  • 03:44Differentiation of Monocytes into Macrophages
  • 04:25Preparation of Biological Samples for MALDI-TOF MS
  • 05:08Whole Cell MALDI-TOF MS Analysis
  • 07:22Bioinformatic Analysis
  • 08:05Results: Macrophage Responsiveness
  • 10:56Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

Tags

Keywords: MALDI-TOFMass SpectrometryMacrophage ActivationWhole-cell AnalysisProteomic FingerprintingCell IdentificationDiagnostic Applications

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