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Chapters
- 00:05Title
- 01:24Embedding Human Bone Marrow Cells in a 3D Culture
- 03:57Isolating Cells from 3D Culture
- 06:37Representative Results: Human BM Cell Growth and Morphology in Reconstructed BM
- 08:16Conclusion
In standard culture methods cells are taken out of their physiological environment and grown on the plastic surface of a dish. To study the behavior of primary human bone marrow cells we created a 3-D culture system where cells are grown under conditions recapitulating the native microenvironment of the tissue.
