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December 30, 2013
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V3 workflow is a western blot procedure using stain-free gels. The stain-free technology allows researchers to visualize protein separation quality, to verify the transfer efficiency, and most importantly, to validate the change in the protein of interest using total protein quantification as a reliable loading control.
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Cite this Article
Posch, A., Kohn, J., Oh, K., Hammond, M., Liu, N. V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting. J. Vis. Exp. (82), e50948, doi:10.3791/50948 (2013).
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