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Immunology and Infection

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Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Summary
Automatic Translation

This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.

Tags

Isolation Myeloid Dendritic Cells Epithelial Cells Human Thymus Protocol Antigen Presenting Cells Microenvironments Thymic Selection Characterization Low Frequency Efficient Procedure Reproducible Cell Suspension Percoll Density Centrifugation Myeloid DC CD11c+ Magnetic Cell Sorting TEC Populations MTEC CTEC Depletion Of Hematopoietic Cells Fluorescence Activated Cell Sorting (FACS) Downstream Applications
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