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Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)
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Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

Article DOI: 10.3791/50968-v • 10:36 min • December 5th, 2013
December 5th, 2013 •
1, 1, 1, 1
1Neuroscience Center of Excellence, Louisiana State University Health Sciences Center

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Drosophila is famous for its powerful genetic manipulation, but not for its suitability of in-depth biochemical analysis. Here we present a TAP-based procedure to identify interacting partners of any protein of interest from the fly brain. This procedure can potentially lead to new avenues of research.

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Protein-protein Interaction Drosophila Melanogaster Tandem Affinity Purification (TAP) Genetic Screens Biochemical Screens GAL4/UAS System Bait Protein Fly Neurons Purification Mass Spectrometry Candidate Proteins Loss-of-function Studies Fly Model System Neurobiology
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