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Autonomt Bioluminescent pattedvrceller for vedvarende og Real-time overvågning af cytotoksicitet
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JoVE Journal Biology
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomt Bioluminescent pattedvrceller for vedvarende og Real-time overvågning af cytotoksicitet

DOI:
10.3791/50972-v

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04:47 min

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October 28, 2013

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, , , ,
1The Joint Institute for Biological Sciences,Oak Ridge National Laboratory, 2490 BioTech, Inc., 3The Center for Environmental Biotechnology,The University of Tennessee, Knoxville

Chapters

  • 00:05Title
  • 01:11Cell Plating and Experimental Treatment
  • 02:22Imaging and Data Analysis
  • 03:06Results: Representative Cellular Bioluminescence Dynamics in Response to Chemical Exposure
  • 04:15Conclusion

Summary

Automatic Translation

Automatic Translation

Pattedyrceller udtrykker bakterielle bioluminescens genkassette (<em> Lux</em>) Producere lys selvstændigt. De resulterende bioluminescerende dynamik upon kemiske eksponering har vist sig at afspejle effekten af ​​behandlingen på cellulær vækst og metabolisme, hvilket gør disse celler en billig, vedvarende, real-time toksicitet screeningsværktøj, der nemt kan tilpasses til high-throughput automatisering.

Tags

Autonomously BioluminescentMammalian CellsCytotoxicity MonitoringLux Gene CassetteReal-timeNon-destructiveIn Vitro AssayToxicological StudiesCell-based AssayOptical SignalBiosentinelCellular Metabolism

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