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JoVE Journal
Bioengineering

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Isolation of Cellular Lipid Droplets
 

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Article doi: 10.3791/50981
April 1st, 2014

Summary April 1st, 2014

Two techniques for isolating cellular lipid droplets from 1) yeast cells and 2) human placentas are presented. The centerpiece of both procedures is density gradient centrifugation, where the resulting floating layer containing the droplets can be readily visualized by eye, extracted, and quantified by Western Blot analysis for purity.

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