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内质网子域的培养细胞中的可视化
JoVE Journal
Biology
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JoVE Journal
Biology
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
内质网子域的培养细胞中的可视化
DOI:
10.3791/50985-v
•
16:43 min
•
February 18, 2014
•
Matteo Fossati
2,3
,
Nica Borgese
3,4
,
Sara Francesca Colombo
3
,
Maura Francolini
2
1
Fondazione Filarete
,
2
Department of Biotechnology and Translational Medicine
,
University of Milan
,
3
Neuroscience Institute
,
National Research Council (CNR)
,
4
Department of Health Science
,
“Magna Graecia” University of Catanzaro
Chapters
00:05
Title
01:37
Plasmid, Cell Culture, and Transfection with ER Fluorescent Proteins
03:21
Live Fluorescence Scanning Confocal Microscopy
08:35
Ultrastructural Analysis by Means of Transmission Electron Microscopy
12:38
Representative Imaging Results
16:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了使用调查转染荧光蛋白通过活细胞的共聚焦成像装置驻留在内质网(ER)的分布和流动的成像方法。我们还超微结构分析其表达对这个亚细胞区室的结构的效果。
Tags
Endoplasmic Reticulum
Cell Compartments
Protein Trafficking
Lipid Mobility
FRAP
FLIP
Live-cell Imaging
Fluorescence Microscopy
Electron Microscopy
Organelle Subcompartments
Physiological Conditions
Pathological Conditions
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