Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Quantitative Optical Microscopy: Measurement of…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Quantitative Optical Microscopy
Tissue Engineering of the Intestine in a Murine Model
Journal (Bioengineering)
Tissue Engineering of the Intestine in a Murine Model
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
Journal (Bioengineering)
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
 

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Article DOI: 10.3791/50988-v • 14:09 min • April 7th, 2014
April 7th, 2014 •
1,2, 1, 1,3
1Department of Biomedical Engineering, Oregon Health & Science University, School of Medicine, 2Department of Dermatology, Oregon Health & Science University, School of Medicine, 3Department of Cell & Developmental Biology, Division of Hematology & Medical Oncology, Knight Cancer Institute, Oregon Health & Science University, School of Medicine

Chapters

Summary
Automatic Translation

We describe the use of a standard optical microscope to perform quantitative measurements of cellular mass, volume, and density through a combination of bright field and differential interference contrast imagery.

Tags

Quantitative Optical Microscopy Cellular Biophysical Features Standard Optical Microscope Bright Field Imagery Differential Interference Contrast Imagery Noninterferometric Quantitative Phase Microscopy (NIQPM) Hilbert Transform Differential Interference Contrast Microscopy (HTDIC) Total Cell Mass Subcellular Density Distribution Volume Measurement Wave Propagation Model Paraxial Approximation Numerical Aperture (NA) Illumination Weak Scattering Weak Absorption Unstained Cellular Specimens Low NA Illumination High NA Illumination Through-focus DIC Imagery Hilbert Transform Processing Edge Detection Algorithms Three-dimensional Localization Advantages Of NIQPM And HTDIC
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter