Journal
/
Bioengineering
/
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

DOI:
10.3791/50988-v

•

14:09 min

•

April 07, 2014

•

, ,
1Department of Biomedical Engineering,Oregon Health & Science University, School of Medicine, 2Department of Dermatology,Oregon Health & Science University, School of Medicine, 3Department of Cell & Developmental Biology, Division of Hematology & Medical Oncology, Knight Cancer Institute,Oregon Health & Science University, School of Medicine

Chapters

  • 00:05Title
  • 01:29Differential Interference Contrast Z-stack Acquisition
  • 03:16Volume Measurements
  • 07:54Mass Measurements
  • 09:56Results: Biophysical Cell Measurements
  • 13:33Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of a standard optical microscope to perform quantitative measurements of cellular mass, volume, and density through a combination of bright field and differential interference contrast imagery.

Tags

Keywords: Quantitative Optical MicroscopyCellular Biophysical FeaturesBright FieldDifferential Interference ContrastNoninterferometric Quantitative Phase MicroscopyNIQPMHilbert Transform Differential Interference Contrast MicroscopyHTDICParaxial ApproximationLow Numerical ApertureWeak ScatteringWeak AbsorptionVolumetric InformationHigh NA IlluminationEdge DetectionDiffraction Effects

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50988?article_type=v

Preparation of Complaint Matrices for Quantifying Cellular Contraction
check_url/50988?article_type=v

Three-dimensional Optical-resolution Photoacoustic Microscopy
check_url/50988?article_type=v

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
check_url/50988?article_type=v

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
check_url/50988?article_type=v

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
check_url/50988?article_type=v

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
check_url/50988?article_type=v

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
check_url/50988?article_type=v

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
check_url/50988?article_type=v

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
check_url/50988?article_type=v

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Read Article