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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Article DOI: 10.3791/51018
December 13th, 2013 •
1, 1, 1, 1
1Ophthalmology, Weill Medical College of Cornell University

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December 13th, 2013

The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.

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