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从胚胎前脑内皮细胞的分离和培养
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
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从胚胎前脑内皮细胞的分离和培养

DOI:
10.3791/51021-v

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12:02 min

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January 23, 2014

•

,
1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Chapters

  • 00:05Title
  • 01:45Removal of Embryos and Dissection of Telencephalon
  • 02:53Cell Isolation
  • 04:43Magnetic Labeling and Separation
  • 07:26Cultured Periventricular Endothelial Cell Characterization
  • 11:24Conclusion

Summary

Automatic Translation

Automatic Translation

该视频演示了一个简单而可靠的战略准备内皮细胞从内10-12天的胚胎前脑的纯培养物，并将成为研究主要集中在脑血管的许多方面非常有用。

Tags

Keywords: Embryonic ForebrainEndothelial CellsPial Vascular NetworkPeriventricular Vascular NetworkIsolationCulturePurificationCD31/PECAM-1AngiogenesisNeurovascular Development

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