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荧光<em>原位</em>杂交(FISH)技术对病毒和植物和昆虫组织内共生细菌的本土化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
DOI:

10:38 min

February 24, 2014

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Chapters

  • 00:05Title
  • 01:26Whitefly, Plant, and Virus Preparations
  • 02:10Whole Mount FISH of Whiteflies
  • 03:58Whitefly Dissection Followed by TYLCV FISH
  • 06:40Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
  • 08:29Results: FISH Images
  • 10:16Conclusion

Summary

Automatic Translation

我们在这里描述原位杂交简单的荧光(FISH)方法对病毒和细菌的昆虫和植物组织的定位。这个协议可以延长mRNA的整装可视化和显微切片。

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