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Journal
/
Immunology and Infection
/
荧光<em>原位</em>杂交(FISH)技术对病毒和植物和昆虫组织内共生细菌的本土化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Fluorescence
in situ
Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
Please note that all translations are automatically generated.
Click here for the English version.
荧光<em>原位</em>杂交(FISH)技术对病毒和植物和昆虫组织内共生细菌的本土化
DOI:
10.3791/51030-v
•
10:38 min
•
February 24, 2014
•
Adi Kliot
2
,
Svetlana Kontsedalov
,
Galina Lebedev
,
Marina Brumin
,
Pakkianathan Britto Cathrin
,
Julio Massaharu Marubayashi
,
Marisa Skaljac
3
,
Eduard Belausov
,
Henryk Czosnek
,
Murad Ghanim
1
Department of Entomology
,
Volcani Center
,
2
Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment
,
Hebrew University of Jerusalem
,
3
Department of Applied Sciences
,
Institute for Adriatic Crops and Karst Reclamation
,
4
The Institute of Plant Sciences
,
Volcani Center
Chapters
00:05
Title
01:26
Whitefly, Plant, and Virus Preparations
02:10
Whole Mount FISH of Whiteflies
03:58
Whitefly Dissection Followed by TYLCV FISH
06:40
Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
08:29
Results: FISH Images
10:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述
原位
杂交简单的荧光(FISH)方法对病毒和细菌的昆虫和植物组织的定位。这个协议可以延长mRNA的整装可视化和显微切片。
Tags
Fluorescence In Situ Hybridization (FISH)
Virus Localization
Endosymbiotic Bacteria
Spatial Localization
Visualization
Infection Process
Reporter Gene Systems
Immunohistochemical Methods
RNA/DNA Probe
Fixation
Hybridization
Microscopic Visualization
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