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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
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Neuroscience
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JoVE Journal Neuroscience
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

DOI:
10.3791/51070-v

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09:07 min

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March 17, 2014

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, , ,
1Cellular and Molelcular Neurobiology,Max Planck Institute of Experimental Medicine, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Chapters

  • 00:05Title
  • 01:26Transfection of Cultured Neurons
  • 04:20In Vivo Electroporation
  • 07:10Results: Genetic Manipulation of Cerebellar Granule Neurons to Study Neuronal Morphology and Migration
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

Neuronal morphogenesis and migration are crucial events underlying proper brain development. Here, we describe methods to genetically manipulate cultured cerebellar granule neurons and the developing cerebellum for the assessment of morphology and migratory characteristics of neurons.

Tags

Keywords: Cerebellar Granule NeuronsGenetic ManipulationNeuronal MorphogenesisNeuronal MigrationIn Vitro AnalysisIn Vivo AnalysisAxonogenesisDendritogenesisRNA InterferenceOverexpressionSmall MoleculesRodent Cerebellar CortexIn Vivo Electroporation

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