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Aplysia Ganglia Preparation for Electrophysiological and Molecular Analyses of Single Neurons
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Chapters
- 00:05Title
- 01:32Preparation of Abdominal Ganglia
- 02:54Electrophysiological Measurements and Isolation of Single Identified Neurons
- 04:09RNA Isolation from Single Neurons and Gene Expression Analysis by Quantitative Real Time PCR
- 07:01Results: Electrophysiological and Molecular Analyses of Single Neurons of Aplysia Ganglia
- 08:08Conclusion
Marine snail Aplysia californica has been widely used as a neurobiology model for the studies on cellular and molecular basis of behavior. Here a methodology is described for exploring the nervous system of Aplysia for the electrophysiological and molecular analyses of single neurons of identified neural circuitry.
