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Bezeichnung des Post-translationale Modifikationen von Pflanzenproteinkomplexe
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Bezeichnung des Post-translationale Modifikationen von Pflanzenproteinkomplexe

Article DOI: 10.3791/51095-v • 10:07 min • February 22nd, 2014
February 22nd, 2014 •
1, 2, 2, 1,2, 3, 1
1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University

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Hier beschreiben wir ein Protokoll für die Reinigung und Charakterisierung von Pflanzenproteinkomplexen. Wir zeigen, dass durch Immunpräzipitation ein einzelnes Protein in einem Komplex, so können wir seine post-translationale Modifikationen und seinen Interaktionspartner zu identifizieren.

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Plant Biology Ausgabe 84 Pflanzen-Mikroben-Interaktionen Protein-Komplex Reinigung Massenspektrometrie Protein-Phosphorylierung Prf Zapfwelle AvrPto AvrPtoB
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