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JoVE Journal
Immunology and Infection

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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
 

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Article doi: 10.3791/51135
April 9th, 2014

Summary April 9th, 2014

High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.

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