15,374 Views
•
11:56 min
•
February 12, 2014
DOI:
Summary
Automatically generated
We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.
Related Videos
09:21
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
Related Videos
23834 Views
06:35
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
Related Videos
54303 Views
11:48
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
Related Videos
34962 Views
06:39
Live Imaging of Dorsal Root Axons after Rhizotomy
Related Videos
13757 Views
09:52
Inducing Dendritic Growth in Cultured Sympathetic Neurons
Related Videos
12678 Views
06:55
Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
Related Videos
15539 Views
11:58
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
Related Videos
82939 Views
13:50
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
Related Videos
17008 Views
11:10
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
Related Videos
13651 Views
08:15
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Related Videos
7994 Views
Read Article
Cite this Article
Carrodus, N. L., Teng, K. S., Munro, K. M., Kennedy, M. J., Gunnersen, J. M. Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons. J. Vis. Exp. (84), e51139, doi:10.3791/51139 (2014).
Copy