Journal
/
Medicine
/
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

DOI:
10.3791/51171-v

•

07:13 min

•

March 26, 2014

•

, , , , , , , , , ,
1Tumor Microenvironment and Metastasis Program,Wistar Institute, 2Department of Pathology and Lab Medicine, Perelman School of Medicine,University of Pennsylvania, 3Department of Microbiology and Immunology and Department of Genetics,Geisel School of Medicine at Dartmouth, 4Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine,University of Pennsylvania, 5Rena Rowan Breast Center, Abramson Cancer Center,University of Pennsylvania, 6Center for Advanced Medicine,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:13Intraductal Injection of Virus Particles
  • 04:00Results: Representative Tumor Formation and Metastasis
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Tags

Keywords: Metastatic Breast CarcinomaTransgenic Mouse ModelDuctal EpitheliumAdenovirus-creP53 AblationK-ras OncogeneTumor ProgressionLymphovascular InvasionLeukocyte InfiltrationLatent MetastasisDormancyImmunotherapy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51171?article_type=v

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth
check_url/51171?article_type=v

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
check_url/51171?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/51171?article_type=v

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI
check_url/51171?article_type=v

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
check_url/51171?article_type=v

An Ex vivo Model to Study Hormone Action in the Human Breast
check_url/51171?article_type=v

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
check_url/51171?article_type=v

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
check_url/51171?article_type=v

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
check_url/51171?article_type=v

Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior

Read Article