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In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
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In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

DOI:
10.3791/51187-v

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09:04 min

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May 02, 2014

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, , , , , , , ,
1Division of Hematology-Oncology, Department of Internal Medicine,University of Cincinnati College of Medicine, 2Division of Human Genetics,University of Cincinnati College of Medicine, 3Department of Radiology,University of Cincinnati College of Medicine, 4Division of Rheumatology, Cincinnati Children’s Hospital Medical Center,University of Cincinnati College of Medicine, 5Solid Tumor Biology Program, James Comprehensive Cancer Center,The Ohio State University Medical Center, 6Department of Neurological Surgery, James Comprehensive Cancer Center,The Ohio State University Medical Center

Chapters

  • 00:05Title
  • 01:25Preparation of Animal Models and Fluorescently-labeled SapC-DOPS Nanovesicles
  • 03:27Fluorescence Imaging
  • 05:30Results: Optical Imaging of Brain Tumors and Arthritis in SapC-DOPS Nanovesicle Injected Mice
  • 08:35Conculsion

Summary

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Automatic Translation

We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.

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Keywords: In Vivo Optical ImagingFluorescent NanovesiclesSapC-DOPSBrain TumorArthritisMulti-angle Rotational Optical Imaging (MAROI)Quantitative Analysis

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