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In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Article DOI: 10.3791/51187 • 09:04 min • May 2nd, 2014
May 2nd, 2014 •
1,2, 3, 1, 5,6, 5,6, 4, 4, 3, 1,2
1Division of Hematology-Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine, 2Division of Human Genetics, University of Cincinnati College of Medicine, 3Department of Radiology, University of Cincinnati College of Medicine, 4Division of Rheumatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 5Solid Tumor Biology Program, James Comprehensive Cancer Center, The Ohio State University Medical Center, 6Department of Neurological Surgery, James Comprehensive Cancer Center, The Ohio State University Medical Center

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Summary
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We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.

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In Vivo Optical Imaging Brain Tumors Arthritis Fluorescent Marker SapC-DOPS Nanovesicles MAROI System Physiopathological Processes Fluorescent Fluorophore Intravenous Administration Rotational Holder In Vivo Imaging System Mean Fluorescence Intensity Background Subtraction Orthotopic Brain Tumors Transgenic Brain Tumors Arthritic Sites Curve Analysis Optimal Angle For Imaging Noninvasive Imaging Quantitative Analysis
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