Optical Imaging System
Optical Imaging System - Related Videos
16:44
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
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Cited by 15 •
2009
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
07:46
Optical Materialography Part 2: Image Analysis
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2023
Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA The imaging of microscopic structures of solid materials, and the analysis of the structural components imaged, is known as materialography. Often, we would like to quantify the internal three-dimensional microstructure of a material using only the structural features evidenced by an exposed two-dimensional surface. While X-ray based tomographical methods can reveal buried...
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
FAQs
The image depends on whether the system illuminates the specimen and records transmitted light, or captures light emitted by the specimen. Lenses then focus and magnify the collected signal, while a detector converts it into digital data. Choosing between transmitted and emitted light allows researchers to examine different structural or activity-related features without directly contacting the biological sample.
Filters and contrast methods help isolate particular features from the broader optical signal. In biological experiments, they can separate signals associated with fluorescent labels, making selected structures or activities easier to distinguish in the recorded image. This selectivity supports clearer examination of specimens and provides a basis for analyzing specific biological features rather than treating the entire image as uniform.
Focusing places the relevant specimen features into a usable image plane, while magnification enlarges the collected signal for examination. Together, these lens functions allow researchers to inspect biological organization across different scales, from cells to tissues and developing organs. Their effect is especially important when the goal is to compare structural features or follow changes within a specimen.
Structural information can come from light transmitted through or reflected by the specimen, whereas activity may be represented by emitted light, including signals from fluorescent labels. By selecting the appropriate illumination, collection pathway, filters, and contrast approach, an Optical Imaging System can record complementary aspects of a sample. This supports studies that connect visible organization with biological responses or dynamic behavior.
A typical workflow begins by positioning the biological specimen for optical observation, then illuminating it or collecting light it emits or transmits. Lenses focus and magnify the signal, and filters or contrast methods are applied when particular features must be isolated. A detector records the result as digital data, which can then support image examination, quantitative analysis, or tracking.
These systems are useful when investigators need to observe biological processes without direct contact and follow changes over time. The overview identifies live-cell tracking and organ development as important uses, allowing researchers to examine activity, organization, and progression across relevant scales. The resulting images can also help evaluate how biological specimens respond during an experiment.
Recorded images can support both descriptive observation and quantitative analysis. Researchers may examine cellular or tissue structure, track living cells, follow organ development, and identify disease-related changes. Because the detector converts optical signals into digital data, image-based measurements can be compared across specimens or experimental conditions to evaluate biological responses and changes over time.
By allowing researchers to visualize cells and tissues without direct contact, the system can reveal disease-related changes and document responses in biological specimens. Imaging may combine structural observation with signals from fluorescent labels or other contrast methods, while digital records support comparison and quantitative analysis. These capabilities make optical imaging useful for connecting visible changes with experimental biological responses.