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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Double Negative αβ T Cells from the Kidney
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Isolation of Double Negative αβ T Cells from the Kidney

Article DOI: 10.3791/51192-v • 06:56 min • May 16th, 2014
May 16th, 2014 •
1, 2, 2, 1
1Department of Pathology, Johns Hopkins University School of Medicine, 2Department of Medicine, Johns Hopkins University School of Medicine

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Summary
Automatic Translation

DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

Tags

Isolation Double Negative T Cells 945 946 Kidney Protocol Non-lymphoid Tissues Immune Responses Immune Cell Type Autoimmune Responses Tolerance Allotransplants Peripheral Blood Lymphoid Organs Spleen Lymph Nodes Pathophysiologic Function Phenotypic Analysis Functional Analysis Ischemia Reperfusion Injury Pure Ab CD4+ CD8+ T Cells Murine Kidney Collagenase Digestion Kidney Mononuclear Cells (KMNC) Density Gradient Hematopoietic Cells Positive Selection CD45+ Isolation Kit Negative Isolation CD4 CD8 MHC Class II Monoclonal Antibodies
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