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JoVE Journal
Immunology and Infection

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Isolation of Double Negative αβ T Cells from the Kidney
 

Isolation of Double Negative αβ T Cells from the Kidney

Article DOI: 10.3791/51192
May 16th, 2014

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Summary May 16th, 2014

DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

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