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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av Double Negative αβ T-celler från njure
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Isolering av Double Negative αβ T-celler från njure

Article DOI: 10.3791/51192-v • 06:56 min • May 16th, 2014
May 16th, 2014 •
1, 2, 2, 1
1Department of Pathology, Johns Hopkins University School of Medicine, 2Department of Medicine, Johns Hopkins University School of Medicine

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DNabT celler är sällsynta bland perifera T-celler; Men, de är rikligt förekommande i vissa icke-lymfoida vävnader. Svårighet att isolera DN T-celler från icke-lymfatisk vävnad hindrar deras funktionella analyser trots ökande erkänd patofysiologiska betydelse. Vi beskriver en roman protokoll för isolering av höggradigt renade DN T-celler från mus njure.

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Immunologi Double Negative (DN) αβ T-celler CD45 + T-cell isolering njur lymfocyter icke-lymfoida-vävnader T-celler rening ischemireperfusionsskada akut njurskada Tissue Resident Lymfocyter lymfoproliferativa sjukdomar Erythematosus Lupus
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