Journal

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

DOI:

10.3791/51208-v

•

04:25 min

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February 24, 2014

•

Maha Habib, Barbara D. Porter, Catherine Satzke²

¹Pneumococcal Research,Murdoch Childrens Research Institute, ²Department of Microbiology & Immunology,The University of Melbourne

Chapters

00:05Title
01:12Preparing the Cell Suspension for Typing
02:29Typing
03:03Results: Representative Negative and Positive Quelling Reactions
03:32Conclusion

Summary

Automatic Translation

The Quellung reaction is the gold standard technique for serotyping Streptococcus pneumoniae. This technique utilizes a microscope and specific pneumococcal antisera and is commonly used in reference and research laboratories worldwide.

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