Serological Typing
Serological Typing - Related Videos
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
05:25
Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
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2023
A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.
10:07
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
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Cited by 11 •
2017
This work describes a FACS-based protocol that allows for easy and simultaneous isolation of type I and type II pericytes from skeletal muscles.
FAQs
The distinguishing signal comes from which antibodies bind to antigenic markers on the tested microorganism or cell. Related variants may share some markers but differ in others, producing different reaction combinations. Interpreting the complete pattern, rather than relying on a single binding event, allows researchers to classify isolates and separate antigenically similar but nonidentical groups.
Agglutination provides a visible readout of antibody binding: antibodies link particles carrying compatible antigenic markers, causing them to form detectable aggregates. The presence or absence of this reaction across a panel of specific antibodies creates a characteristic profile. That profile can then support serotype assignment and comparison among samples examined during infection studies.
Antigenic variation changes the markers displayed by related isolates, so their antibody reactivity can differ even when they are connected biologically or epidemiologically. Serological typing captures this variation as distinct reaction patterns. Mapping those differences helps researchers compare strains, recognize distributions of antigenic types, and determine when molecular analyses may provide useful complementary information.
A typical workflow exposes the microorganism or cell sample to antibodies with known specificity and records the resulting binding reactions. Observations such as agglutination are organized into a serological pattern, which is compared with the patterns associated with recognized serotypes. The resulting classification depends on the combined reaction profile rather than on one observation alone.
Researchers apply the method when they need to distinguish related variants among isolates collected during infection studies. Comparing serological profiles can support pathogen identification, strain comparison, and outbreak investigation. It also contributes to epidemiological surveillance by showing how antigenic types are distributed across the samples or settings being examined.
The method reveals which antigenic markers and serological types occur among examined isolates. That information can help researchers compare circulating variants with the antigenic features relevant to vaccine evaluation and examine relationships among samples during transmission tracking. Because the method focuses on antigenic patterns, molecular analyses may be selected alongside it when additional strain resolution is needed.