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JoVE Journal
Bioengineering

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Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
 

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Article DOI: 10.3791/51215
June 3rd, 2014

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Summary June 3rd, 2014

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

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