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A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
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Neuroscience
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JoVE Journal Neuroscience
A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

DOI:
10.3791/51219-v

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09:37 min

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June 15, 2014

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, ,
1Regenerative Medicine Institute,Cedars-Sinai Medical Center

Chapters

  • 00:05Title
  • 01:36hNPC Observations
  • 02:13Chopper Setup
  • 03:22Pre-chop Procedure
  • 05:58Chop Procedure
  • 07:08Post-chop Procedure
  • 08:11Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a novel mechanical chopping method that allows the expansion of spherical neural stem and progenitor cell aggregates without dissociation to a single cell suspension.  Maintaining cell/cell contact allows rapid and stable growth for over 40 passages.

Tags

Keywords: CGMPExpansion MethodNeural Stem CellsNeural Progenitor CellsPluripotent Stem CellsNeurospheresCell CultureMechanical PassagingAutomated ChoppingSuspension CultureCell YieldScale-upClinical-grade Cell Production

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