A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:59Selection and Orientation
- 02:27Preparing for Imaging
- 03:30Microscopy
- 04:43Analysis and Results
- 05:43Conclusion
This paper demonstrates the use of a fast scanning confocal microscope to image cell behavior directly through the puparium. By leaving the pupal case intact, this method allows observation and measurement of dynamic cell processes at a stage of Drosophila development that is difficult to study directly.
